Antibody identification art or science a case study approach answer key

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What makes an antibody positive or negative in a cell?

• Pattern – Most or all cells positive, autocontrol negative – 1 or 2 cells positive, autocontrol negative – Panreactivity – Variability—an antibody showing dosage effect, multiple antibodies, or antigen showing variable expression from one panel cell to another – Weak, variable reactivity • Physical Appearance

When does an antibody have a clinical significance?

An antibody is considered significant if it has been associated with –HDFN –HTR –Notable decreased survival of RBCs –The degree of clinical significance varies among antibodies with the same specificity •Most commonly identified alloantibodies: –Anti-D –Anti-E –Anti-K Clinical Significance and Prevalence Albumin LISS PeG Gel Solid Phase

Who are the scientists in advanced antibody identification?

Advanced Antibody Identification: Case Studies • Thank you to the following scientists at the ARUP Immunohematology Reference Lab (IRL) for contributing to several of the case studies used in this presentation: – Rebecca Whitney – Catherine Thom – Noel Pusey – Becky Condas 1. Sally Rudmann, Ed.

Are there antibodies to low incidence antigens in blood?

• Antibodies to low incidence antigens are rarely seen (although most people can form the antibody, the antigens are rarely found on donor blood—antibodies usually formed through HDFN) –Cw, Kpa, Jsa, Lua, etc. Knowledge of Antibody Specificities

Last Update: Oct 2021


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